FIGURE 2 from The TERT Promoter is Polycomb-Repressed in Neuroblastoma Cells with Long Telomeres
Year: 2024
Type: other
Abstract: Molecular characterization of telomere maintenance in neuroblastoma cell lines. Telomere maintenance mechanism was assessed in eight neuroblastoma cell lines used in this study: MYCN-amplified (NB5, SKNBE2, IMR32), moderate level of MYCN without amplification (NBL-S), ATRX-mutant (SKNMM, CHLA90), low-DAXX (SKNFI) and ever-shorter telomere (LAN6) neuroblastoma cells. A, Representative images of Telomere FISH (green) showing brighter signal in cell lines with long telomeres. B, Telomeric repeats in neuroblastoma cell lines relative to those in the telomerase-positive 293T cells using qPCR. Telomerase-negative, ALT-positive U2OS cells were used as a positive control for long telomeres. C, Representative blot of C-circle assay. U2OS cells were used as a positive control and PC3 cells were used as a negative control. The amount of DNA in nanograms (ng) is indicated on the blot. All reactions were done in the presence or absence of the Phi 29 (Phi) DNA polymerase. qRT-PCR was performed in neuroblastoma cell lines to measure TERT (D) and TERC (E) expression relative to the telomerase-positive 293T cell line. Gene expression was normalized to HPRT expression. The U2OS cell line was used as an ALT-positive control and does not express TERC.
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4399855682