FIGURE 3 from Activation of the Mevalonate Pathway in Response to Anti-cancer Treatments Drives Glioblastoma Recurrences Through Activation of Rac-1
Year: 2024
Type: other
Abstract: Statins reduce treatment-induced upregulation of cholesterol biosynthesis in PDOXs. A, Brain and plasma levels of atorvastatin in C57BL/6 mice after a single injection (atorvastatin – 30 mg/kg, i.p.). B, Schematic of the experimental design underlying Fig. 3. C, The levels of total, free cholesterol and cholesterol-esters in the tumors harvested from the PDOX GBM mouse model at day 5 after the treatment of radiation in combination with QTP (30 mg/kg), atorvastatin (30 mg/kg) or simvastatin (7 mg/kg). D, The concentration of free fatty acid in the tumors harvested from the PDOX GBM mouse model at day 5 after the treatments. E, Heat map showing the results of qRT-PCR for the cholesterol biosynthesis–related genes in the tumor specimen harvested from the PDOX GBM mouse model at day 5 after the treatments using the human- and mouse-specific primers. F, Survival curves for NSG mice implanted intracranially with 3 × 105 HK374-GFP-Luciferase glioma cells and grafted for 3 days. Mice were irradiated and treated with saline or QTP (30 mg/kg, s.c., 5-day on/2-day off schedule) or triple combination of radiation plus QTP and simvastatin (7 mg/kg, i.p., 5-day on/2-day off schedule) continuously until they reached the study endpoint. log-rank (Mantel–Cox) test for comparison of Kaplan–Meier survival curves. G, Weight curves for the NSG mice in different treatment groups. All experiments have been performed with at least three biological independent repeats. P-values were calculated using one-way ANOVA. ns: not significant.
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4400002188