Differential expression of cellular prion protein (PrPC) in mouse hepatitis virus induced neuroinflammation
Year: 2024
Type: article
Abstract: The cellular prion protein (PrPC) is an extracellular cell membrane protein. Due to its diversified roles, a definite role of PrPC has been difficult to establish. During viral infection, PrPC has been reported to play a pleiotropic role. Here, we have attempted to envision the function of PrPC in the neurotropic m-CoV-MHV-RSA59-induced model of neuroinflammation in C57BL/6 mice. A significant upregulation of PrPC at protein and mRNA levels was evident in infected mouse brains during the acute phase of neuroinflammation. Furthermore, investigation of the effect of MHV-RSA59 infection on prion expression in specific neuronal, microglial, and astrocytoma cell lines, revealed a differential expression of prion during neuroinflammation. Additionally, siRNA-mediated downregulation of prnp transcripts reduced the expression of viral antigen and viral infectivity in these cell lines. Cumulatively, our results suggest that PrPC expression significantly increases during acute MHV-RSA59 infection and that PrPC also assists in viral infectivity and viral replication.
Source: Journal of NeuroVirology
Authors: Satavisha Ghosh, Rishika Jana, Soumen Jana, Rahul Basu, Madhurima Chatterjee, Nishtha Ranawat, Jayasri Das Sarma
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: National Multiple Sclerosis Society, Indian Institute of Science, Multiple Sclerosis Society, Department of Biotechnology, Ministry of Science and Technology, India, University Grants Commission, Indian Institute of Science Education and Research Mohali, Indian Institute of Science Education and Research Kolkata
OpenAlex ID: W4400039536