Protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage
Year: 2024
Type: article
Abstract: Electroporation is a technique to introduce DNA constructs into cells using electric current. Here, we present a protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage. We describe steps for setting up and conducting electroporation. We then detail procedures for collecting, fixing, and mounting embryos and counting expression. This protocol can be used to study the expression of enhancers via reporter assays, manipulating cells using genes or modified genes such as dominant negatives, and genome editing. For complete details on the use and execution of this protocol, please refer to Song, et al.1
Source: STAR Protocols
Institution: University of California San Diego
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funders: National Science Foundation, American Heart Association, Hartwell Foundation, National Institutes of Health, University of California, San Diego, National Heart, Lung, and Blood Institute, National Human Genome Research Institute, National Institute of General Medical Sciences, NHLBI Division of Intramural Research
Awards: G2857399215, G3910713819, Elucidation of causal variants contributing to CHD and arrhythmias, Deciphering the regulatory principles governing enhancer specificity, G645488662, Elucidating regulatory principles governing heart development, NSF Postdoctoral Fellowship in Biology FY 2021: Deciphering grammatical rules of notochord enhancers and their role in notochord development in Ciona intestinalis, Training in Cardiovascular Physiology and Pharmacology, Pathways in Biological Sciences Training Program
OpenAlex ID: W4400279938