Figure 3 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Year: 2024
Type: other
Abstract: Gli2 is required for the regulation of Ccnd1 downstream of oncogenic KRAS. A, Heatmap of GLI target gene expression in 1012U cells at 12, 24, 48, and 72 hours posttreatment with doxycycline (Dox). B, Relative gene expression of Ccnd1 by qPCR in mouse pancreas tissue from Cre, CRG, KC, and KCRG mice. C, Western blot showing expression of p-ERK, total ERK, and CCND1 in 1012U −Dox and +Dox cells at 12, 24, 48, and 72 hours post treatment. Vinculin is used as the loading control. D, Relative gene expression of Ccnd1 and Gli2 by qPCR after the knockdown of Gli2 by siRNA in 1012U cells +Dox cells (72 hours treatment; P < 0.05 and P < 0.001). E, Western blot (left) and protein quantification (right) representing expression of CCDN1 and GLI2 in 1012U cells +Dox, after the knockdown of Gli2. Vinculin is used as the loading control. F, Western blot (left) and protein quantification (right) representing expression of CCDN1 and GLI2 in 1012U cells +Dox and murine KC cell line transfected with Gli2, ∆NGli2, or empty vector (control). Vinculin is used as the loading control. G, Top: Graphical representative of mouse Ccnd1 promoter region with (red) diamond indicating candidate Gli2-binding site. Bottom: Graph representing enrichment (percent input) of GLI2 at the Ccnd1 promoter in 1012U +Dox cells (72 hours treatment; P < 0.05).
Authors: Ashley N. Sigafoos, Ezequiel J. Tolosa, Ryan M. Carr, Maite G. Fernandez-Barrena, Luciana L. Almada, David R. Pease, Tara L. Hogenson, Glancis L. Raja Arul, Fatemeh Mousavi, Sandhya Sen, Renzo E. Vera, David L. Marks, Luis F. Flores, Kayla C. LaRue-Nolan, Chen Wu, William R. Bamlet, Anne M. Vrabel, Hugues Sicotte, Erin L. Schenk, Thomas C. Smyrk, Lizhi Zhang, Kari G. Rabe, Ann L. Oberg, Peter G. Zaphiropoulos, Eric Chevet, Rondell P. Graham, Catherine E. Hagen, Marina P. di Magliano, Sherine F. Elsawa, Christopher L. Pin, Junhao Mao, Robert R. McWilliams, Martin E. Fernandez-Zapico
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4400458663