Figure 4 from KRAS Promotes GLI2-Dependent Transcription during Pancreatic Carcinogenesis
Year: 2024
Type: other
Abstract: Gli2 drives H3K4me3 enrichment at Ccnd1 promoter in mutant KRAS cells. A, Heatmaps representing global levels of H3K4me3 enrichment in 1012U −Dox and 1012U +Dox cells. B, MA-plot highlighting differentially enriched H3K4me3 peaks in the 1012U +Dox cells compared with the 1012U −Dox cells. C, Profile plot of H3K4me3 at the transcriptional start site + or − 5 kb comparing 1012U +Dox cells with the 1012U −Dox cells. D, ChIP-seq tracks showing enrichment of H3K4me3 in 1012U –Dox and +Dox cells for GLI target gene Ccnd1. E, Western blot (left) and protein quantification (right) representing expression of H3K4me3 in 1012U cells +Dox. Total H3 is used as the loading control. F, H3K4me3 enrichment at Ccdn1 promoter in 1012U +Dox cells postknockdown of Gli2.
Authors: Ashley N. Sigafoos, Ezequiel J. Tolosa, Ryan M. Carr, Maite G. Fernandez-Barrena, Luciana L. Almada, David R. Pease, Tara L. Hogenson, Glancis L. Raja Arul, Fatemeh Mousavi, Sandhya Sen, Renzo E. Vera, David L. Marks, Luis F. Flores, Kayla C. LaRue-Nolan, Chen Wu, William R. Bamlet, Anne M. Vrabel, Hugues Sicotte, Erin L. Schenk, Thomas C. Smyrk, Lizhi Zhang, Kari G. Rabe, Ann L. Oberg, Peter G. Zaphiropoulos, Eric Chevet, Rondell P. Graham, Catherine E. Hagen, Marina P. di Magliano, Sherine F. Elsawa, Christopher L. Pin, Junhao Mao, Robert R. McWilliams, Martin E. Fernandez-Zapico
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4400458692