Work

sciMET-cap: high-throughput single-cell methylation analysis with a reduced sequencing burden

Year: 2024

Type: article

Abstract: DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-wide nature of DNA methylation results in a high sequencing burden per cell. Here, we leverage target enrichment with sciMET to capture sufficient information per cell for cell type assignment using substantially fewer sequence reads (sciMET-cap). Accumulated off-target coverage enables genome-wide differentially methylated region (DMR) calling for clusters with as few as 115 cells. We characterize sciMET-cap on human PBMCs and brain (middle frontal gyrus).

Source: Genome biology

DOI: 10.1186/s13059-024-03306-7

Authors: Sonia N. Acharya, Ruth V. Nichols, Lauren E. Rylaarsdam, Brendan L. O’Connell, Theodore P. Braun, Andrew C. Adey

Institutions: Oregon Health & Science University, OHSU Knight Cancer Institute

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,290

Funders: Oregon Health and Science University, Silver Family Foundation, National Institutes of Health, National Institute of Mental Health