Year: 2025
Type: article
Abstract: Circadian disruption enhances cancer risk, and many tumors exhibit disordered circadian gene expression. We show rhythmic gene expression is unexpectedly robust in clear cell renal cell carcinoma (ccRCC). The core circadian transcription factor BMAL1 is closely related to ARNT, and we show that BMAL1-HIF2α regulates a subset of HIF2α target genes in ccRCC cells. Depletion of BMAL1 selectively reduces HIF2α chromatin association and target gene expression and reduces ccRCC growth in culture and in xenografts. Analysis of pre-existing data reveals higher BMAL1 in patient-derived xenografts that are sensitive to growth suppression by a HIF2α antagonist (PT2399). BMAL1-HIF2α is more sensitive than ARNT-HIF2α is to suppression by PT2399, and the effectiveness of PT2399 for suppressing xenograft tumor growth in vivo depends on the time of day at which it is delivered. Together, these findings indicate that an alternate HIF2α heterodimer containing the circadian partner BMAL1 influences HIF2α activity, growth, and sensitivity to HIF2α antagonist drugs in ccRCC cells.
Source: Nature Communications
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: U.S. Department of Health and Human Services, Deutscher Akademischer Austauschdienst, Deutsche Forschungsgemeinschaft, National Institutes of Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences
Awards: The San Diego Regional Network Award for Kidney, Urologic, and Hematologic Research Training, G1426848669, G1812447635, G1914974694, G2270087404, G2595524682, G2755953110, G3027042729, G3715906717, TRR 333: Braunes und beiges Fett Organinteraktionen, Signalwege und Energiehaushalt (BATenergy), Scripps Translational Science Institute, G8318251302, Establishing a mechanistic basis for enhanced tumorigenesis under chronic circadian disruption, Metabolic Influences on Complex Tumor Neighborhoods, CIRCADIAN REGULATION OF HIF2alpha IN ccRCC
OpenAlex ID: W4411846906