Work

From mother's milk to structural insights: 1H–15N NMR analysis of Lewis X antigen-bearing oligosaccharides isolated from human milk

Year: 2024

Type: article

Abstract: Human milk oligosaccharides (HMOs) are complex unconjugated glycans abundant in breast milk, with vital roles in infant nutrition and health. The structural elucidation of HMOs remains challenging due to their diverse compositions and isomeric complexities. This study presents a novel approach employing 1 H– 15 N NMR correlations to pinpoint and characterize HMOs, LNH, LNnH, and their fucosylated derivatives including those of bearing the Lewis X motif. Isolated from human milk, these hexa-, hepta-, and octasaccharides were extensively analysed by NMR. This enabled their first complete 1 H, 13 C and 15 N resonance assignments . Using 1 H– 15 N HSQC experiments, the distinctive 1 H– 15 N correlations of GlcNAc units within the HMOs provided structural fingerprints, allowing for unequivocal differentiation of isomeric structures and fucosylation patterns. Results demonstrate the potential of 1 H– 15 N NMR spectroscopy in decoding complex HMO structures, offering new perspectives on their detailed structural characterization and contributing to a deeper understanding of their potential benefits.

Source: Carbohydrate Polymers

DOI: 10.1016/j.carbpol.2024.122534

Authors: Zsófia Garádi, András Dancsó, Vladimir Piskarev, Szabolcs Béni

Institutions: Semmelweis University, Egis Pharmaceuticals (Hungary), Russian Academy of Sciences, A. N. Nesmeyanov Institute of Organoelement Compounds, Eötvös Loránd University

Language: English

Topic: Infant Nutrition and Health

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $5,010