Figure 3 from N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
Year: 2024
Type: other
Abstract: NMT inhibition increases lipid peroxidation and induces caspase-independent cell death in (KL/K)MUT lung carcinoma cells. A, Lipid peroxidation measured using Bodipy 581/591 C11 and flow cytometry in (KL/K)MUT and (KL/K)WT lung carcinoma cells treated with 1 μmol/L DDD85646 (NMTi) or vehicle control (DMSO) for 96 hours. Erastin (10 μmol/L for 24 hours) was used as positive control. Green fluorescence (oxidized probe) was normalized to control DMSO. n = 2 independent experiments. MFI, mean fluorescence intensity. B, Cell viability (Live/dead reagent) was analyzed in (KL/K)MUT cells treated for 72 hours with vehicle control or 1 μmol/L DDD85646 (NMTi) in the presence or absence of 25-μmol/L ZVAD-FMK added freshly every 24 hours. Graph: percentage of dead cells calculated from at least 5,000 cells per condition. Bar, group mean, error bars, SEM. ****, P < 0.0001; ns, not significant (Student t test). Representative images are shown. Bar, 100 μm. C, Cell viability (live/dead reagent) analyzed in (KL/K)MUT cells treated for 72 hours with vehicle control or 1 μmol/L DDD85646 (NMTi) in the presence or absence of the ferroptosis inhibitor liproxstatin (5 and 10 μmol/L). Graph: percentage of dead cells calculated from at least 5,500 cells per condition. Crossbar, group mean; error bars, SEM. *, P < 0.05; ns, not significant (Student t test). Representative images are shown. Bar, 100 μm.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4400980329