Quantification of nisin concentration from fluorescence‐based antimicrobial activity assay using Bayesian calibration
Year: 2024
Type: article
Abstract: Bacteriocins are ribosomally synthesized peptides with the innate ability to kill or inhibit growth of other bacteria. In recent years, bacteriocins have received increased interest, as their antimicrobial activity enhances food safety and shelf life by combatting pathogens such as Listeria monocytogenes. They also have application potential as an active pharmaceutical compound to combat multidrug-resistant pathogens. As new bacteriocins continue to be discovered, accelerated workflows for screening, identification, and process development have been developed. However, antimicrobial activity measurement is often still limited with regards to quantification and throughput. Here, we present the use of a non-linear calibration model to infer nisin concentrations in cultivation supernatants of Lactococcus lactis ssp. lactis B1629 using readouts of pHluorin2 fluorescence-based antimicrobial activity assays.
Source: Biotechnology Progress
DOI: 10.1002/btpr.3495
Institutions: Forschungszentrum Jülich, RWTH Aachen University, Universität Ulm
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,160
Awards: Maßgeschneiderte Inhaltsstoffe 2 - Verbundvorhaben: 'Pipeline und Zellfabriken zur Identifikation und Produktion neuer antimikrobieller Peptide (AMPLIFY) - Teilprojekt A', Maßgeschneiderte Inhaltsstoffe 2 - Verbundvorhaben: 'Pipeline und Zellfabriken zur Identifikation und Produktion neuer antimikrobieller Peptide (AMPLIFY) - Teilprojekt C'
OpenAlex ID: W4401029604