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Optimization of Plant Growth Regulators in Indirect Plant Regeneration in Rice Variety Nerica 3 Using Mature Seeds as Explant

Year: 2024

Type: article

Abstract: The study is the first attempt to highlight about callus induction and plantlet regeneration in rice variety ‘Nerica 3’ using mature seed as explant and using five regeneration media with plant growth regulators (BA, IAA, and 2,4-D) at different concentrations in other to establish the efficient indirect plant regeneration protocol. Among the five regeneration media tested, R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%), whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%). These results will be used for genetic improvement programs of the variety ‘Nerica 3’.

Source: Biotechnology Journal International

DOI: 10.9734/bji/2024/v28i4733

Authors: Danielle Christelle Tinak Ekom, Haïcha Diko Abba, Jean Cyrille Mouen Piau, Hermine Claudine Mahot, Nathalie Gaelle Tinak Satok, Rodrigue Simonet Poueme Namegni, Abel Wade

Institutions: Institut de Recherche Agricole pour le Développement, Ministry of Livestock, Fisheries and Animal Industries, Centre Pasteur du Cameroun

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W4401031778

FWCI:

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