Work

OHSU SenNet Senescence-Associated Beta-Galactosidase (SA b-Gal) Staining of Carboxymethyl Cellulose (CMC) Embedded Skin Tissue Sections on Polyethylene Naphthalate (PEN) Coated Slides v1

Year: 2024

Type: preprint

Abstract: Senescence-associated beta-galactosidase staining is a common staining technique to detect senescent cells. This protocol describes SA b-Gal staining of mouse skin tissue sections from CMC embedded blocks onto PEN slides which enable the combined use of laser capture microdissection (LCM) and UV laser cutting for downstream mass spectrometry analysis.

DOI: 10.17504/protocols.io.81wgbzq7ygpk/v1

Authors: Julia R Unsworth, Xianmin Luo, Sheila A. Stewart, Megan K. Ruhland

Institutions: Oregon Health & Science University, Washington University in St. Louis, Alvin J. Siteman Cancer Center

Language: English

Topic: Telomeres, Telomerase, and Senescence

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4401105874

Cites:

Cited by:

Related to: 10