Work

Nanodisc assembly from bacterial total lipid extracts

Year: 2024

Type: article

Abstract: Nanodiscs are discoidal lipoproteins that have often been used as vehicles to study membrane proteins in their native configuration. Nanodiscs have been primarily made from synthetic lipids. However, nanodiscs also offer a format by which native lipids can be studied in their natural configuration. Here, we present a method to synthesize nanodiscs from bacterial total lipid extracts using the biothreat agent, Yersinia pestis, as a proof-of-concept. The creation of nanoparticles entirely composed of bacterial lipids supports membrane characterization and vaccine antigen discovery without the inherent safety concerns associated with live bacterial cells of this Tier 1 select agent pathogen.

Source: Chemistry and Physics of Lipids

DOI: 10.1016/j.chemphyslip.2024.105425

Authors: Trent R. Llewellyn, Olivia R.C. Pimentel, Kiersten D. Lenz, Makaela M. Montoya, Jessica Z. Kubicek-Sutherland

Institution: Los Alamos National Laboratory

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,760