Work

Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition

Year: 2024

Type: article

Abstract: Besnoitia besnoiti is a cyst-forming apicomplexan parasite and the causal agent of bovine besnoitiosis. During early phase of infection, tachyzoites replicate within host endothelial cells in a host cell cholesterol-dependent process. By applying U18666A treatments, we here evaluated the role of Niemann–Pick type C protein 1 (NPC1) in both, intracellular B. besnoiti replication and host cellular cholesterol distribution. Additionally, B. besnoiti-driven changes in NPC1 gene transcription were studied by qPCR. Overall, U18666A treatments significantly reduced B. besnoiti proliferation and induced cholesterol accumulation in host cytoplasmic dense vesicles. However, NPC1 gene transcription was not affected by B. besnoiti infection.

Source: Frontiers in Veterinary Science

DOI: 10.3389/fvets.2024.1454855

Authors: Camilo Larrazabal, Carlos Hermosilla, Anja Taubert, Liliana M. R. Silva

Institutions: Justus-Liebig-Universität Gießen, Universidad Católica de Temuco, University of Évora, Escola Superior de Saúde Egas Moniz, Centro de Investigação Interdisciplinar Egas Moniz

Language: English

Topic: Toxoplasma gondii Research Studies

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,295