Nucleating Agent Crystallophore Induces Instant Protein Crystallization
Year: 2024
Type: article
Abstract: ABSTRACT The rapid preparation of homogeneous suspensions of micro- or nano-crystals is a crucial step in serial crystallography. We show how additives, such as the crystallophore (TbXo4) that acts as a molecular glue by promoting protein-protein interactions, can facilitate sample preparation for both serial synchrotron crystallography (SSX) and micro electron diffraction (3D ED). This lanthanide complex was used here for its nucleating properties to crystallize hen egg white lysozyme. SAXS monitoring indicates that crystals formed in a few minutes in low salt conditions that would not lead to spontaneous nucleation. Resulting micro- and nano-crystals were successfully used to determine the structure of the lysozyme-TbXo4 complex by SSX and 3D ED, illustrating the diffraction quality of the produced crystals and the usefulness of such compounds in the sample preparation pipeline for serial crystallography.
Source: Crystal Growth & Design
Institutions: Centre National de la Recherche Scientifique, Architecture et Réactivité de l'arN, Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biologie Structurale, Université Grenoble Alpes, European Synchrotron Radiation Facility, European X-Ray Free-Electron Laser, European Molecular Biology Laboratory, Synchrotron SOLEIL, École Normale Supérieure de Lyon, Laboratoire de Chimie
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4401459897