Tree-dimensional structure of the fluorescent complex of bacterial lipocalin triple mutant with chromophore – DiB3-F53L/F74L/L129M
Year: 2024
Type: article
Abstract: The three dimensional structure of DiB3-F53L/F74L/L129M – a triple mutant of the fluorescent non-covalent complex of a genetically engineered variant of the bacterial protein lipocalin Blc with the synthetic GFP-like chromophore M739, has been studied by molecular dynamics methods. It was found that the chromophore binding site in the complex is similar to DiB1 and differs from the alternative site in DiB3. The complex is characterized by increased fluorescence brightness compared to those with other genetically engineered variants of lipocalin, which makes it one of the promising markers of biological objects in cell biology.
Source: Биоорганическая химия
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4401493211