Guidelines for validating analytical methods for ginsenoside Rg3, Rk1, Rg5 from black ginseng
Year: 2024
Type: article
Abstract: This study was conducted to establish the manufacturing and quality control of standards for black ginseng and propose a standard analytical method to present marker components different from red ginseng.Changes in the content of ginsenosides Rg3(s), Rk1, and Rg5 were investigated according to the number of steaming and drying processes of black ginseng.In the industrially manufactured black ginseng, the sum of Rg3(s), Rk1, and Rg5 was more than 3 mg/g.In addition, through Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry analysis-based metabolomic analysis, ginsenosides Rg3(s), Rk1, and Rg5 of black ginseng were identified as components differentiated from red ginseng in the results of multivariate statistical analysis and metabolic profiling.The analytical method was validated through HPLC to standardize the marker components.For method validation, the analytical method was tested for specificity, linearity, accuracy, precision, limit of detection, and limit of quantitation, and it was found to be suitable for standardization of marker components.Panax)에 속하는 다년생 초본으로서 한국의 대표적인 약 용작물이다[1].인삼은 한의학적으로 중요한 보기약(補氣藥) 중 의 하나이며, 다양한 한의서 및 중의서에서 체력증진, 소화기, 신경, 대사, 순환기 계통 등의 기능 조절을 위한 처방 약재로 사용되어 왔다[2,3].최근에는 코로나19 팬데믹 이후로 건강기 능식품으로써 면역기능의 중요한 인식으로, 인삼류 제품은 우리 나라뿐만 아니라 전 세계적으로 관심이 높아졌다.「인삼산업 법」 에 고시하고 있는 인삼류는 수삼, 백삼, 태극삼, 홍삼으로 분류되고 있으며 백삼은 수삼을 햇볕 또는 열풍으로 건조시킨 것이고, 태극삼은 수삼을 뜨거운 물에 일정시간 넣어 익혀 말 린 것이며, 홍삼은 수삼을 수증기 또는 기타 방법으로 쪄서 익 혀 건조한 것을 말한다[4].
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Funder: Rural Development Administration
Award: G2337239961
OpenAlex ID: W4401500844