Evaluation of inhibitory action of bacteriophage against Multi-drug resistant Escherichia coli
Year: 2024
Type: article
Abstract: Background: Bacteriophages are ubiquitous in the environment where their host resides such as rivers, soil, sewage, poultry, or animal feces, and seawater. thus, the present study aims to isolate the multidrug-resistant (MDR) Escherichia coli (E. coli) from various clinical specimens of patients and to inhibit these MDR E. coli by bacteriophage. Methodology: A Hospital-based cross-sectional study was carried out in the Microbiology laboratory of Star Hospital, Sanepa, Lalitpur from June- August 2021. 175 Clinical isolates of E. coli positive culture of E. coli and bacteriophage against E. Coli. were taken for Isolation of bacteriophage (plaque assay) using standard microbiological methods and Isolation of bacterial strains followed by purification of phages and determination of host spot test. Ethical consideration was taken from the IRC of Star Hospital Research Council. The data was entered in Ms. Excel 2013 and analyzed by SPSS version 17. Results: Out of 3 sewages, 2 phages were isolated named as $E against Escherichia coli. Phages showed positive results against Escherichia coli and $E phage was found to be large, and clear from plaque assay. Total 70 E. coli was tested against 10 antibiotics out of which 37(52.9%) were MDR E. coli and 33(47.1%) were non MDR E. coli. Among 70 samples of E. coli, 38(54.3%) showed to be sensitive and 32(45.7%) showed to be resistant to isolated bacteriophage. Among 37 MDR E. coli isolates, 17(44.7%) were found to be sensitive and 33 Non-MDR E. coli, 21(55.3%) were found to be sensitive. Conclusion: Bacteriophages can be used to treat the infection caused by E. coli which are MDR.
Authors: Sumi Budhathoki, Susma Kandel, Dibbya Chand, Saroj Kunwar, Govinda Akela Poudel, Ram Krishna Shrestha, Ashok Kumar Shah, Suresh Jaiswal, Rajesh Kumar Thakur, Rupa Nepal
Institutions: Pokhara University, Galgotias University
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4401501122