Cost-effective scanning digital microscopic system for wide field-of-view and high-resolution biomedical imaging
Year: 2024
Type: conference-paper
Abstract: Microscopes serve as indispensable tools for exploring the microcosm and conducting biological detection. However, traditional microscopes fail to balance the field of view and resolution, limiting wide-field imaging capabilities. Here, we propose a low-cost, wide-field, high-resolution digital microscopy imaging system, providing high-quality and versatile microscopy tools for budget-constrained laboratories. The design of this system is based on the low-cost mechanical scanning and positioning platform provided by a computer numerical control (CNC) router architecture. The use of a transmitted light structure ensures uniform field illumination for both bright-field and fluorescence microscopy. Utilizing a 20× objective lens, this microscopy system achieves clear imaging of 1μm particles and rapidly acquires hundreds of microscopic images for subsequent stitching, thus achieving high-resolution wide-field imaging in both bright-field and fluorescence modes. The system integrates the advantages of wide-field, high-resolution imaging, and high-throughput image acquisition, providing a cost-effective solution for wide-field microscopy.
Authors: Siru Lin, Zhihang Lv, Xiwei Huang
Institution: Hangzhou Dianzi University
Language: English
Subfield: Media Technology
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4401526397