Work

osm-5p-driven fluorophores are differentially expressed in ccpp-1Δ and nekl-4Δ mutant ciliated neurons

Year: 2024

Type: article

Abstract: Intraflagellar transport (IFT) involves the coordinated transport of molecular motors and other proteins and is required for ciliogenesis and ciliary maintenance. The C. elegans IFT protein OSM-5/IFT88 is expressed in a majority of the ciliated neurons in the animal, and osm-5 mutants exhibit structurally defective cilia. The osm-5 promoter is commonly used to express genetic constructs in the ciliated neurons. In this study, we show that brightness of osm-5p-driven constructs is altered in mutants of the tubulin deglutamylase ccpp-1 and the NIMA-related kinase nekl-4. This raises the possibility that osm-5 expression levels may be regulated by ccpp-1 and nekl-4.

Source: PubMed

DOI: 10.17912/micropub.biology.001245

Authors: Kaiden M Power, Maureen M Barr

Institution: Rutgers, The State University of New Jersey

Language: English

Topic: Genetic and Kidney Cyst Diseases

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke