Figure 3 from Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4
Year: 2024
Type: other
Abstract: Plasma membrane permeabilization and mitochondrial changes caused by Acetalax. A, Representative flow cytometry data showing plasma membrane permeabilization induced by Acetalax within 5 minutes as measured by PI assays. Acetalax concentrations are color-coded as indicated. The x-axis shows PI absorbance, and the y-axis shows cell counts. The right shifts of the peaks in the MDA-MB468, BT549, and Hs578T sensitive cell lines indicate increased permeability. B, Flow cytometric mitochondrial membrane potential (MT-1) assay. Acetalax treatments were at 1 μmol/L for 5 and 60 minutes. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (2 μmol/L for 60 minutes) was used as the positive control. The x-axis shows mitochondrial membrane potential, and the y-axis shows cell counts. The left shifts of the peaks seen in the sensitive cell lines indicate decreased mitochondrial membrane potential. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, an uncoupler of oxidative phosphorylation, was used as the positive control. C, ATP depletion. Cells were treated with Acetalax 60, 120, and 240 minutes at 1 μmol/L. ATP depletion was calculated as the percentage change compared with the untreated group as measured by CellTiter-Glo 2. D, Mitochondrial fragmentation. Cells were treated with Acetalax for 4 hours at 10 μmol/L. Fragmentation volume percentage was calculated as the total fragmented surface volume divided by the total mitochondrial volume per cell. Percentage of fragmented mitochondria on the y-axis represents the comparison between control and Acetalax-treated groups. A, Significant change in mitochondrial fragmentation is indicated by the brackets. *, P < 0.01; **, P < 0.005; ***, P < 0.0001 (using the Mann–Whitney U test, calculated in Prism 9.0). Center lines indicate medians. FCCP, Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone.
Language: English
Topic: Ion Channels and Receptors
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4401584856