Hybrid Amyloid Quantum Dot Nano-Bio Assemblies to Probe Neuroinflammatory Damage
Year: 2024
Type: article
Abstract: Various oligomeric species of amyloid-beta have been proposed to play different immunogenic roles in the cellular pathology of Alzheimer's Disease. The dynamic interconversion between various amyloid oligomers and fibrillar assemblies makes it difficult to elucidate the role each potential aggregation state may play in driving neuroinflammatory and neurodegenerative pathology. The ability to identify the amyloid species that are key and essential drivers of these pathological hallmarks of Alzheimer's Disease is of fundamental importance for also understanding downstream events including tauopathies that mediate neuroinflammation with neurologic deficits. Here, we report the design and construction of a quantum dot mimetic for larger spherical oligomeric amyloid species as an "endogenously" fluorescent proxy for this cytotoxic assembly of amyloid to investigate its role in inducing inflammatory and stress response states in neuronal and glial cell types. The design parameters and construction protocol developed here may be adapted for developing quantum dot nano-bio assemblies for other biological systems of interest, particularly neurodegenerative diseases involving other protein aggregates.
Source: ACS Chemical Neuroscience
Authors: Wesley Chiang, Jennifer M. Urban, Francine Yanchik-Slade, Angela Stout, Jennetta M. Hammond, Bradley L. Nilsson, Harris A. Gelbard, Todd D. Krauss
Institutions: University of Rochester Medicine, University of Rochester
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Funders: National Science Foundation, University of Rochester, National Institutes of Health, Center for AIDS Research, University of Washington, Medical Center, University of Rochester, National Heart, Lung, and Blood Institute, National Institute of Mental Health, NIH Office of the Director, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, Division of Materials Research
Awards: G216383997, G2309442529, G2556149400, G2803885226, CAREER: Amyloid-Inspired Self-Assembled Hydrogel Materials for Cell Culture Applications, G4617330678, G508620783, G5657816298, Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF/TOF) Mass Spectrometer, Major Mental Disorders from Childhood to Adulthood, G8108324397, G8477475712, G9000003064, G9000174477, G9000260491, University of Rochester Center for AIDS Research
OpenAlex ID: W4401608847