A Single Interfacial Point Mutation Rescues Solution Structure Determination of the Complex of HMG‐D with a DNA Bulge
Year: 2024
Type: article
Abstract: Broadening of signals from atoms at interfaces can often be a limiting factor in applying solution NMR to the structure determination of complexes. Common contributors to such problems include exchange between free and bound states and the increased molecular weight of complexes relative to the free components, but another cause that can be more difficult to deal with occurs when conformational dynamics within the interface takes place at an intermediate rate on the chemical shift timescale. In this work we show how a carefully chosen mutation in the protein HMG‐D rescued such a situation, making possible high‐resolution structure determination of its complex with a dA2 bulge DNA ligand designed to mimic a natural DNA bend, and thereby revealing a new spatial organization of the complex.
Source: ChemBioChem
Authors: Guy R. Hill, Ji‐Chun Yang, Laura E. Easton, Rachel Cerdan, Stephen H. McLaughlin, Katherine Stott, Andrew A. Travers, David Neuhaus
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,220
OpenAlex ID: W4401615563