INF2-mediated actin polymerization at ER-organelle contacts regulates organelle size and movement
Year: 2024
Type: preprint
Abstract: Proper regulation of organelle dynamics and inter-organelle contacts is critical for cellular health and function. Both the endoplasmic reticulum (ER) and actin cytoskeleton are known to regulate organelle dynamics, but how, when, and where these two subcellular components are coordinated to control organelle dynamics remains unclear. Here, we show that ER-associated actin consistently marks mitochondrial, endosomal, and lysosomal fission sites. We also show that actin polymerization by the ER-anchored isoform of the formin protein INF2 is a key regulator of the morphology and mobility of these organelles. Together, our findings establish a mechanism by which INF2-mediated polymerization of ER-associated actin at ER-organelle contacts regulates organelle dynamics.
Source: Research Square
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Funders: National Science Foundation, American Heart Association, Charcot-Marie-Tooth Association, Waitt Foundation, National Institutes of Health, University of California, San Diego, National Institute on Aging, National Eye Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke, National Institute of Arthritis and Musculoskeletal and Skin Diseases
Awards: NeuroNex: Enabling Identification and Impact of Synaptic Weight in Functional Networks, San Diego Nathan Shock Center, Actin-mediated regulation of organelle dynamics in Charcot-Marie-Tooth disease, G3749142063, G4066625888, G4801963049, G6015879438, Mitochondrial DNA Stress Activation of Interferon Signaling and Lupus Pathology, G7852963419
OpenAlex ID: W4401627191