Charge Detection Mass Spectrometry Enables Molecular Characterization of Nucleic Acid Nanoparticles
Year: 2024
Type: article
Abstract: Nucleic acid nanoparticles (NANPs) are increasingly used in preclinical investigations as delivery vectors. Tools that can characterize assembly and assess quality will accelerate their development and clinical translation. Standard techniques used to characterize NANPs, like gel electrophoresis, lack the resolution for precise characterization. Here, we introduce the use of charge detection mass spectrometry (CD-MS) to characterize these materials. Using this technique, we determined the mass of NANPs varying in size, shape, and molecular mass, NANPs varying in production quality due to formulations lacking component oligonucleotides, and NANPs functionalized with protein and nucleic acid-based secondary molecules. Based on these demonstrations, CD-MS is a promising tool to precisely characterize NANPs, enabling more precise assessments of the manufacturing and processing of these materials.
Source: ACS Nano
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funders: National Science Foundation, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Environmental Health Sciences, Army Research Office, National Science Foundation Graduate Research Fellowship Program
Awards: G1148400999, G1860077899, G2408041277, G3491862500, G4774430914, G4950536974, G5108852245, G5551941966, G613990440, G6274897657, G7275046751, G7452299184, G7861274045, Investigation of Synthetic DNA-based Viral Particles for Spatially Controlled Antigen Presentation, G8727049869, G9000030699, G9000219151, G9000300833, Cancer Center Support (Core) Grant, G941982536
OpenAlex ID: W4401632618