Exploring astrocyte morphological changes under shear stress: a quantum imaging and laser-induced shockwaves approach
Year: 2024
Type: conference-abstract
Abstract: Traumatic Brain Injury (TBI) is the result of external forces impacting the brain. Despite scientific progress, TBI remains a significant cause of impairment and mortality. Recently, laser-induced shockwave (LIS) has emerged as an effective method for TBI simulation. LIS generates shockwaves through pulsed laser-induced plasma formation, allowing for the controlled study of TBI at the cellular level. This study introduces a novel approach to examine cellular morphological changes in response to shear stress, focusing on astrocyte cell type AST-1, by combining LIS with quantitative phase microscopy (QPM). QPM is a label-free technique that allows for real-time cellular dynamics observation through 3D imaging. Integrating LIS and QPM assesses astrocyte responses to shear stress caused by LIS, revealing both immediate and sustained morphological changes. Post-LIS exposure analysis shows significant alterations in astrocyte circularity, volume, surface area, and other features. Statistical tests confirm these observed trends, providing valuable insights into astrocyte responses to mechanical forces. These findings enhance our understanding of how mechanical stimuli affect astrocyte morphology, which may offer the potential for identifying and developing therapeutic strategies in TBI and related neurological disorders.
DOI: 10.1117/12.3028932
Authors: Janelle Ho, Pegah Pouladian, Daryl Preece, Nicole Wakida, Nicolas Perez, Veronica Gomez
Institution: University of California, Irvine
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: green
Awards: G1072749480, G1523888516, G260750634
OpenAlex ID: W4401632619