Year: 2024
Type: article
Abstract: Imaging and characterizing the dynamics of cellular adhesion in blood samples is of fundamental importance in understanding biological function. In vitro microscopy methods are widely used for this task but typically require diluting the blood with a buffer to allow for transmission of light. However, whole blood provides crucial signaling cues that influence adhesion dynamics, which means that conventional approaches lack the full physiological complexity of living microvasculature. We can reliably image cell interactions in microfluidic channels during whole blood flow by motion blur microscopy (MBM) in vitro and automate image analysis using machine learning. MBM provides a low cost, easy to implement alternative to intravital microscopy, for rapid data generation where understanding cell interactions, adhesion, and motility is crucial. MBM is generalizable to studies of various diseases, including cancer, blood disorders, thrombosis, inflammatory and autoimmune diseases, as well as providing rich datasets for theoretical modeling of adhesion dynamics.
Source: Nature Communications
Institution: Case Western Reserve University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,790
Funders: National Science Foundation, U.S. Department of Health and Human Services, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Cleveland Clinic, Case Western Reserve University, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine, Case Western Reserve University, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases
Awards: G1405401648, G1984980855, STTR Phase II: Optimized manufacturing and machine learning based automation of Endothelium-on-a-chip microfluidic devices for drug screening applications., G2338498481, G269954282, Microfluidic Technology for Concurrent Assessment of Red Blood Cell Adhesion and Deformability, CAREER: Molecular heterogeneity and the regulation of cell adhesion by force, Endothelialized microfluidic assays for emerging therapies in sickle cell disease, G6482406840, G661630905, Roles of Red Blood Cell Derived Extracellular Vesicles in Complement Activation and Thromboinflammation in sickle cell disease, STTR Phase I: Standardized and reproducible culture and activation of endothelial cells in microfluidic devices for drug screening applications, CAREER: Biomechanics of Red Blood Cell Adhesion and Deformability, A Modality-Agnostic Potency Assay Enabling Both Ex Vivo and In Vivo Genome Editing Therapeutics for Sickle Cell Disease
OpenAlex ID: W4401643593