Work

Identification of Candida species isolated from cancer patients by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism in Apeksha Hospital, Maharagama, Sri Lanka

Year: 2024

Type: article

Abstract: Candida infections pose a significant challenge in managing cancer patients. A rapid and effective method for Candida species identification is crucial to address this issue. This study at Apeksha Hospital, Maharagama, aimed to identify Candida species using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism. ITS1-5.8S-ITS2 rDNA regions of 52 clinical Candida isolates and standard strains were amplified using ITS1 and ITS4 primers and digested with MspI enzyme. Candida tropicalis (38%), Candida parapsilosis (31%), Candida albicans (13%), Candida glabrata (8%), Candida krusei (4%), Candida haemulonii (4%), and Candida intermedia (2%) were identified. PCRRFLP proves to be a rapid, and reliable method for identifying medically important Candida species in cancer patients.

Source: Ceylon Medical Journal

DOI: 10.4038/cmj.v68i3.9928

Authors: Sammarappulige Chathma Ganguli, Somalatha Wijendra, Nilanthie Dasanayaka, Ramesh Ratnasingham, Gaya Kaushalya, Samanmalee Priyantha Gunasekera

Institutions: University of Sri Jayewardenepura, Medical Research Institute, National Cancer Institute of Sri Lanka

Language: English

Topic: Antifungal resistance and susceptibility

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4401647981