Mechanism study of structural functional dual biomimetic composite bone scaffold for repair of mandibular defects in rabbits
Year: 2024
Type: article
Abstract: The present study aims to explore the potential mechanisms of bone tissue-engineered triply periodic minimal surfaces (TPMS) scaffolds containing injectable platelet-rich fibrin and stromal cell-derived factor-1 (in short, SIT scaffolds) in promoting angiogenesis in mandibular defects. Surface structures with a 70% porosity were created using the Matlab R2020a program. SIT scaffolds were fabricated using digital laser processing. Histological structures on SIT scaffolds inoculated with rabbit bone marrow mesenchymal stem cells (BMSCs) were observed using alkaline phosphatase (ALP) immunohistochemical staining, Alcian blue staining, and Masson-Goldner staining. Double-end sequencing in PE150 mode was performed using the Illumina NovaSeq™ 6000. Analyses such as Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interactions, and gene set enrichment analysis (GSEA) were conducted to delineate the gene functions. RT-qPCR was performed to confirm the expression of genes of interest. Finally, cells on different samples were examined by confocal laser scanning microscopy to observe c-Jun expression. Confocal imaging and quantitative analysis showed that BMSCs supported by the SIT scaffold had a greater tendency to differentiate into osteoblasts than those supported by the TPMS scaffold or the control group. The SIT group exhibited the most intense ALP staining. The expression of angiogenesis-related factors VEGFA was significantly upregulated in T and SIT groups. Expression of osteogenic gene RUNX2 and c-Fos/c-Jun pathway genes FOS/JUN was significantly upregulated in the SIT group. GSEA revealed that the WNT signaling pathway and MAPK signaling pathway were more active in the SIT group. Immunofluorescence showed that the c-Jun is highly expressed in newly formed capillaries in the SIT group. In conclusion, both TPMS and SIT scaffolds promote angiogenesis in mandibular defects.
DOI: 10.36922/ijb.4147
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4401684248