(Co, S)-Codoped BiOBr as a novel signal probe coupled with LAMP (H+)-pH responsive photoelectrochemistry biosensor for ultrasensitive detection of microRNA
Year: 2024
Type: article
Abstract: Herein, Co/S-BiOBr as a novel photoelectric material with excellent photoelectric property was prepared by cation–anion co-doping method with LAMP (H+)-pH responsive as signal amplification to construct a photochemical (PEC) biosensor for ultra-sensitive detection of microRNA-221 that related to triple-negative breast cancer (TNBC). Notably, not only the lattice distortion caused by S doping could elevate the photo-generated electron-hole separation efficiency, but also the tune of electronic structure by Co doping promoted electron transfer and decreased recombination rate, thus significantly improving the photoelectric performance. Furthermore, in the aid of Loop-mediated isothermal amplification (LAMP), a few miRNA-221 could be converted to abundant LAMP(H+) for changing the configuration of the Fc-DNA probe, which made the ferrocene (Fc) approach to electrode via pH-responsive for hindering the transmission of electrons, resulting in a significantly reduced photocurrent signal to enhance the sensitivity of the biosensor. The proposed PEC biosensor exhibited a broad detection range spanning from 1 fM to 10 nM, boasting a detection limit of 0.24 fM. This work prepared a novel material with efficient photoelectric efficiency for the sensitive detection of biomolecules, which demonstrated significant potential in disease diagnosis.
Source: Sensors and Actuators B Chemical
Authors: Meiling Cheng, Hanmei Deng, Menglin Huang, Hongling Li, Ruo Yuan, Shaping Wei
Institution: Southwest University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4401733199