Work

Preliminary evaluation of the neuroprotective activity of Cannabidiol in combination with some current medicinal drugs in vitro

Year: 2024

Type: article

Abstract: Objective: The objective of this study was to evaluate the neurotoxicity and neuroprotective activity of cannabidiol (CBD), valproate, furosemide, metformin, and bilobalide, individually and mixed with CBD in PC12 cells exposed to Glutamate.In addition, antioxidant activity, reactive oxygen species (ROS) production, and caspase-3 activity were evaluated.Methods: Neurotoxicity and neuroprotection were evaluated with the MTT assay; antioxidant activity, and production of ROS with 2,2-diphenyl-1-picryl-hydrazyl-hydrate, and DCFDA methods, respectively; the apoptosis with caspase-3 activity assay.Results: CBD 5 μM, and bilobalide 15.3 μM showed neuroprotective activity by ROS inhibition and decreased caspase-3 activity.Metformin 1548.4 μM did not provide significant neuroprotection but decreased ROS generation.Conclusions: Combining CBD with the study drugs did not improve neuroprotection than the neuroprotection of individual drugs.

Source: Revista de Fomento Social

DOI: 10.24875/rmn.24000002

Authors: Jesús Vélez-Huerta, Eder U. Arredondo-Espinoza, Karla Ramírez-Estrada, Mónica A. Ramírez-Cabrera, Omar González-Santiago

Institution: Universidad Autónoma de Nuevo León

Language: English

Topic: Cannabis and Cannabinoid Research

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Universidad Autónoma de Nuevo León