Work

Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays

Year: 2024

Type: article

Abstract: Aim: -GIC) and a conventional GIC (cGIC) using Ames and Comet assays. Methods: (WP2 uvrA) were employed. The material tested was extracted using sterile distilled water (0.2 g per ml) at 37 °C for 72 h. This was considered as 100 %, which was diluted to 50, 25, 12.5 and 6.25 % utilizing sterile distilled water. These five concentrations were incubated with the bacterial strains with and without metabolic activation (S9), along with appropriate positive controls. The number of revertant colonies was used to evaluate the outcome. Results: -GIC showed less than double the average number of revertant colonies compared to that of negative control with regard to the Ames test. Conclusions: based on the Comet assay.

Source: The Saudi Dental Journal

DOI: 10.1016/j.sdentj.2024.08.009