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Assessing the impact of temperature, pH , light and chemical oxidation on fucoxanthin colour changes, antioxidant activity and the resulting metabolites

Year: 2024

Type: article

Abstract: BACKGROUND The present study evaluated the effects of temperature, pH, light and chemical oxidation on fucoxanthin changes in terms of colour, antioxidant activity and metabolomic profile. Additionally, the correlation between antioxidant activity and identified metabolites was analysed. RESULTS It was found that colour change was significantly reduced at elevated heat (100 °C, *∆E = 0.81 ± 0.05), reduced pH (pH 3, *∆E = 0.59 ± 0.04) and length of light exposure (*∆E = 3.16 ± 0.04). Antioxidant activity decreased under all treatments. Among the temperatures tested, fucoxanthin exhibited the highest activity at 60 °C, ranging from 0.92 to 3.04 mg Trolox equivalents (TE) g−1. Significant activity reductions (P < 0.05) were observed as a result of pH changes in the 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching assays. Exposure to light 2: warm white lamp for 120 h significantly reduced antioxidant activity (0.01 to 1.70 mg TE g−1). Chemical oxidation also led to reduced activity, ranging from 0.18 to 0.29 mg TE g−1. Multivariate data analysis revealed distinct profiles for temperature, pH, light and chemical oxidation treatments. Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS)‐based metabolomics analysis identified 10 metabolites, and significant correlations (P < 0.05) indicate that these metabolites contributed to the samples' antioxidant activities. CONCLUSION In conclusion, fucoxanthin tolerates well at 60 °C, within pH range 3–9, and within 8 h of light exposure, as indicated by its consistent antioxidant activity and minimal colour change. Each treatment resulted in distinct metabolite concentrations, as shown by LC‐MS/MS‐based metabolomics analysis. Further research into these metabolites could advance the understanding of their roles and aid in optimising processing conditions to favour beneficial metabolites. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Source: Journal of the Science of Food and Agriculture

DOI: 10.1002/jsfa.13807

Authors: Zuhaili Yusof, Vuanghao Lim, Nicholas Mun Hoe Khong, Wee Sim Choo, Su Chern Foo

Institutions: Monash University Malaysia, Universiti Sains Malaysia

Language: English

Topic: Phytochemicals and Antioxidant Activities

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $4,400

Funders: Monash University, Monash University Malaysia, School of Science, Monash University Malaysia