Inter-institutional laboratory standardization for SARS-CoV-2 surveillance through wastewater-based epidemiology applied to Mexico City
Year: 2024
Type: article
Abstract: Objectives: Wastewater-based surveillance applied to SARS-CoV-2 viral load quantification for COVID-19 has become one of the most relevant complementary tools in epidemiologic prevention programs worldwide. However, this valuable decision-making tool still requires fine-tuning to produce comparable results between laboratories, especially when applied to the surveillance of megacities. Methods: Six laboratories across Mexico and one from the United States executed an interlaboratory study to set up a singular standardized protocol considering method cost, installed infrastructure, materials available, and supply availability for SARS-CoV-2 quantification from five Mexico City sampling sites across this megacity. Results: Comparable data from processing outcomes in the Mexican laboratories and in the external international laboratory serve as a validating data source. The Bland-Altman comparison showed consistency, with cycle threshold values within ±1.96 SD of SARS-CoV-2 genetic copies for the standard curve quantification, with a mismatch of two laboratories. In addition, MS2 bacteriophage recovery rates varied between 35% and 67% among all participating laboratories. Finally, the efficiency of viral genetic material recovered from all participating laboratories varied between 65% and 93% for the participating laboratories. Conclusion: This work lays the foundation for extensive and continuous wastewater-based surveillance application across independent Mexican laboratories in a time- and resource-effective manner.
Source: IJID Regions
Authors: Mariel Araceli Oyervides-Muñoz, Alberto Aguayo-Acosta, Daniel de los Cobos-Vasconcelos, Julián Carrillo-Reyes, Ana C. Espinosa-García, Eneida Campos, Erin M. Driver, Sofia Liliana Lucero-Saucedo, Arnoldo Armenta-Castro, Orlando de la Rosa, Manuel Martínez-Ruiz, Martín Barragán-Trinidad, Nallely Vázquez-Salvador, Miguel A Silva-Magaña, Marcela Zavala-Méndez, Hafiz M.N. Iqbal, Marisa Mazari-Hiriart, Hugo Velazco, German Buitrón, Adalberto Noyola, Rolf U. Halden, Juan Eduardo Sosa-Hernández, Roberto Parra-Saldívar
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,200
Funders: North Carolina Biotechnology Center, Arizona State University, European Commission, Consejo Nacional de Ciencia y Tecnología, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Instituto Tecnológico y de Estudios Superiores de Monterrey, Secretaría de Estado de Ciencia, Tecnología e Innovación, Sistema Nacional de Investigadores
OpenAlex ID: W4401817163