Work

Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection

Year: 2024

Type: article

Abstract: A miniaturized electrospray interface consisting of a microfluidic nanosprayer and nanospray module is reported in the presented short communication. The nanosprayer was fabricated using silicon (Si) technology suitable for cost-efficient high-volume mass production. The nanospray module enabled the positioning of the nanosprayer in front of a mass spectrometry entrance and its coupling with capillary electrophoresis based on the liquid junction principle. A case study of top-down and bottom-up proteomic analyses of intact cytochrome c and its tryptic digest demonstrates the practical applicability of the developed interface.

Source: Electrophoresis

DOI: 10.1002/elps.202400090

Authors: Tomáš Václavek, Elizaveta Vereshchagina, Leny Nazareno, Anand Summanwar, František Foret, Roman Řemínek

Institutions: Czech Academy of Sciences, Institute of Analytical Chemistry, SINTEF Digital

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,920

Funders: Technology Agency of the Czech Republic, Grantová Agentura České Republiky