A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry
Year: 2024
Type: article
Abstract: Abscisic acid (ABA) is a phytohormone, occurring in plants at very low concentrations (pmol/g fresh weight), that regulates multiple biological processes, including stomatal closure, seed germination and responses to environmental stresses. The isolation of ABA, ABA glucosyl ester and eleven ABA amino acid conjugates in minute quantities of plant tissue (less than 20 mg fresh weight) has been developed using a purification method based on the combination of the Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate was isolated by immunoaffinity chromatography and identified by tandem mass spectrometry in Pisum sativum and Lepidium sativum seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana and water-stressed N. tabacum leaves. In this article, the presence of an ABA conjugate with glutamic acid in plants is described for the first time.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $3,690
Awards: G291036901, G3735356012, G947221780
OpenAlex ID: W4401818683