Work

Naphthopyran-based photoswitching for simpler chemical sensing and imaging using phase-sensitive mode

Year: 2024

Type: article

Abstract: Intensity-based fluorescence imaging suffers from spectral overlap and optical background interference. As an alternative, fluorescence lifetime measurements on the nanosecond level are also largely constrained. Herein, we propose phase-sensitive detection of photoswitchable probes containing naphthopyran and fluorescent donors. The method features reaction kinetics in the millisecond-to-second regime, allowing frequency domain detection with cost-effective equipment. A phase shift (Δϕ) in the fluorescence of the probes is extracted by fast Fourier transform, establishing a dependence on the molar ratio of donor to acceptor. Thus, Δϕ is proposed as a self-referencing quantity for selective highlighting of fluorescent probes and a dynamic signal readout in chemical sensing. Phase-sensitive detection of protamine, a polycationic protein often used as a neutralizer of the anticoagulant heparin during surgery, is successfully realized based on the platform.

Source: Cell Reports Physical Science

DOI: 10.1016/j.xcrp.2024.102167

Authors: Yu Cheng, Yifu Wang, Jingying Zhai, Xiaojiang Xie

Institution: Southern University of Science and Technology

Language: English

Topic: Photochromic and Fluorescence Chemistry

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $5,460

Funders: National Natural Science Foundation of China, Shenzhen Municipal Science and Technology Innovation Council, National Outstanding Youth Science Fund Project of National Natural Science Foundation of China, Science and Technology Planning Project of Shenzhen Municipality