Work

Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models

Year: 2024

Type: article

Abstract: Three-dimensional (3D) ex vivo cultures allow the study of cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring on-target drug sensitivity in a scaffold-free 3D culture system through quantification of apoptotic tumor cells. We provide detailed steps for sample processing, immunofluorescence staining, semi-high-throughput confocal imaging, and imaged-based quantification of 3D cultures. This protocol is versatile and can be applied in principle to any patient-derived material.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103274

Authors: Kathrin B. Labrosse, Flavio C. Lombardo, Natalie Rimmer, Mónica Núñez López, André Fedier, Viola Heinzelmann-Schwarz, Ricardo Coelho, Francis Jacob

Institutions: University of Basel, University Hospital of Basel

Language: English

Topic: Ovarian cancer diagnosis and treatment

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500

Funders: National Science Foundation, Universität Basel, Wilhelm Sander-Stiftung, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Krebsliga Schweiz, Swiss Cancer Research Foundation

Awards: Molecular profiling and drug prediction in ovarian cancer patients, G5280142606, G5495082957, The underestimated role of the human omentum in metastatic spread, G8587610882

OpenAlex ID: W4401824063

FWCI: 1.616

Cites: 9

Cited by: 4

Related to: 10