In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract: Perspectives on Topical Application
Year: 2024
Type: article
Abstract: Biogenic silver nanoparticles (AgNP) are among the fastest-growing nanomaterials due to the simplicity, efficiency, and sustainability of their biosynthesis using phytochemicals as reducing and coating agents. The agro-food industry generates large quantities of organic waste, a renewable source of biomolecules for AgNP biosynthesis. The main objective of this work was to prepare and characterize biogenic AgNP using a green tomato waste extract (TE) obtained by subcritical water extraction. To the best of our knowledge, this is the first report on the use of such an extract in the synthesis of AgNP. The effects of the TE and AgNO3 concentrations, reaction time, pH, and temperature on AgNP physico-chemical characteristics and on in vitro cytotoxicity against HaCaT and THP-1 cells were assessed. Antimicrobial activity was determined in vitro and ex vivo. The wound-healing capability of AgNP was evaluated in vivo in an incisional wound mouse model. The developed AgNP have a Surface Plasmon Resonance (SPR) band between 402 and 406 nm and a size of ±60 nm, and they are negatively charged (−42 mV) and spherical. In vitro and ex vivo studies prove that AgNP do not compromise skin cells and can decrease cutaneous irritation. The AgNP formulated in a gel revealed similar wound-healing properties to a commercial silver-containing topical ointment. Overall, the biogenic synthesis of AgNP employing an extract of agricultural waste obtained by an eco-friendly method is simple and cost-effective and presents the potential for application in skin disease management.
Source: Applied Sciences
DOI: 10.3390/app14167309
Authors: Daniela Cunha, Catarina Faria-Silva, Filomena A. Carvalho, Lia Ascensão, Pedro Simões, Manuela Carvalheiro, Sandra Simões
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,728
Awards: G1169335522, G1240268737, G143066982, G2051880127, G2149047050, G2219685250, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G2637256346, G2736865772, G2756637201, G2814222956, Centro de Estudos do Ambiente e do Mar, G3295750263, G3534458110, Centro de Estudos do Ambiente e do Mar, G4090066734, G4631959511, G4897246272, G5430816075, G587154774, G6119126181, Instituto de Investigação do Medicamento, G670901311, G6996668479, G7508306236, G7665809008, Instituto de Investigação do Medicamento, Centro de Estudos do Ambiente e do Mar, G8805872440, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos
OpenAlex ID: W4401834368