Deciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)
Year: 2024
Type: article
Abstract: Background: Potentilla fulgens, a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine.This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC).Methods: Anti-proliferative effect was assessed by MTT assay.The expression of autophagy and apoptosis-related proteins was evaluated by western blotting.Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods.Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dosedependent manner.MTT assay analysis revealed that the extract significantly (P<0.05)induced mortality in HUVEC, A549, and SKOV-3 cells.Western blot results revealed increased expression of NF-κB after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels.Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group.Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
Institutions: Dicle University, Mardin Artuklu University, Malatya Turgut Özal Üniversitesi, Turgut Özal University, Batman University, Khazar University, Ege University, Saveetha University, Henan University of Science and Technology, Lankaran State University, Baku State University, Ministry of Science and Education Republic of Azerbaijan, Radiasiya Problemləri İnstitutu, King Saud University
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $1,600
Funders: King Saud University, Dicle Üniversitesi
Award: G6576913406
OpenAlex ID: W4401875223