Work

Spatial light modulation for interferometric scattering microscopy

Year: 2024

Type: article

Abstract: Interferometric scattering (iSCAT) microscopy enables high-speed and label-free detection of individual molecules and small nanoparticles. Here we apply point spread function engineering to provide adaptive control of iSCAT images using spatial light modulation. With this approach, we demonstrate improved dynamic spatial filtering, real-time background subtraction, focus control, and signal modulation based on sample orientation.

Source: Journal of Microscopy

DOI: 10.1111/jmi.13347

Authors: Vivien Walter, Christopher Parperis, Yujie Guo, Mark Ian Wallace

Institution: King's College London

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,760

Funders: Wellcome Trust, Oxford Nanopore Technologies, Directorate for Biological Sciences, Biotechnology and Biological Sciences Research Council