Work

Nanoparticle Lysis of Cryptosporidium Oocysts

Year: 2024

Type: article

Abstract: The extraction of DNA from Cryptosporidium oocysts is challenging due to the robust oocyst wall. Nanoparticles have been applied to disinfect Cryptosporidium oocysts; here, we demonstrate the utilisation of nanoparticles to disrupt the oocyst wall to enable sporozoite lysis and detection via PCR. Both silver and zinc oxide nanoparticles are investigated under different conditions and compared to existing techniques. Zinc oxide nanoparticles are shown to be as effective as freeze–thaw methods, suggesting that a nanoparticle lysis approach offers a viable alternative to existing methods.

Source: Methods and Protocols

DOI: 10.3390/mps7050066

Authors: Ameya Vaidya, Claire Bankier, Helinor Johnston, Helen Bridle

Institution: Heriot-Watt University

Language: English

Topic: Parasitic Infections and Diagnostics

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,046