Figure 1 from Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Year: 2024
Type: other
Abstract: Dependency on FOXP3 in IFNγ-mediated PD-L1 expression. A, Expression of FOXP3 and PD-L1 in bladder cancer HT1376, T24, and SW780 cell lines without and with 100 ng/mL IFNγ stimulation by flow cytometry. The mean with SD of at least three independent experiments, P values indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. B, Expression of FOXP3 and PD-L1 in HT1376 CT and HT1376 FOXP3 KO bladder cancer cell lines without and with IFNγ stimulation by qPCR. The mean of triplicates and representative of three independent experiments, P values indicated. C, Expression of FOXP3 and PD-L1 in HT1376 CT and HT1376 FOXP3 KO lines without and with IFNγ stimulation by Western blotting. Sizes in kD as indicated. Sizes of FOXP3Δ3 and FOXP3 depicted by overexpression in 293T cells. Full Western blots shown in Supplementary Fig. S1. D, Flow cytometry of FOXP3 and PD-L1 expression in HT1376 CT and HT1376 FOXP3 KO lines without and with IFNγ stimulation. Representative of four independent experiments. E, Expression of FOXP3 and PD-L1 in rescue of a clonal HT1376 FOXP3 KO line by FOXP3Δ3 and FOXP3 overexpression. Representative of three independent experiments. F, Expression of FOXP3 and PD-L1 in HT1376 FOXP3 KO lines with reconstitution of FOXP3Δ3 and FOXP3 by Western blotting. Sizes in kD as indicated. Full Western blots shown in Supplementary Fig. S1. G, Flow cytometry of PD-L1 in HT1376 CT and HT1376 FOXP3 KO lines stimulated by IFNγ without and with the presence of JAK1/2 inhibitor ruxolitinib (20 μmol/L), P values indicated. H, Expression of phosphorylated STAT1 (P-STAT1) in HT1376 CT and HT1376 FOXP3 KO lines stimulated by IFNγ without and with the presence of JAK1/2 inhibitor ruxolitinib by Western blot. Full Western blots shown in Supplementary Fig. S1.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4401889756