Work

Escherichia coli novel sequence type 11873 harbours a new CTX-M-15–carrying multidrug resistance type 1/2 hybrid IncC plasmid

Year: 2024

Type: article

Abstract: OBJECTIVE: The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15-carrying Escherichia coli strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the E. coli strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing. METHODS: Isolation of E. coli was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two E. coli strains (3-338 and 5-325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing. RESULTS: , qnrS1, tet(A), dfrA14, sul2, strA and strB. CONCLUSIONS: Our study shows the emergence of a CTX-M-15-carrying type 1/2 hybrid IncC plasmid in novel E. coli ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors.

Source: Journal of Global Antimicrobial Resistance

DOI: 10.1016/j.jgar.2024.08.008

Authors: Didrik H. Grevskott, Francisco Salvà-Serra, Edward R.B. Moore, Nachiket P. Marathe

Institutions: Norwegian Institute of Marine Research, Sahlgrenska University Hospital, Universitat de les Illes Balears, University of Gothenburg

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,890