Work

Discovery, Characterization and Synthetic Application of a Promiscuous Nonheme Iron Biocatalyst with Dual Hydroxylase/Desaturase Activity

Year: 2024

Type: article

Abstract: Alpha‐ketoglutarate‐dependent dioxygenases (αKGDs) have recently emerged as useful biocatalysts for C−H oxidation and functionalization. In this work, we characterized a new αKGD from aculene biosynthesis, AneA, which displays broad promiscuity toward a number of substrates with different ring systems. Unexpectedly, AneA was found to be capable of both desaturation and hydroxylation and require an amino ester motif on its substrate for productive catalysis. Insights gathered from the functional characterization and substrate‐activity profiling of AneA enabled the development of a chemoenzymatic strategy toward several complex sesquiterpenoids.

Source: Angewandte Chemie

DOI: 10.1002/ange.202409143

Authors: Hao Xu, Jidong Zhao, Hans Renata

Institution: Rice University

Language: English

Topic: Metal-Catalyzed Oxygenation Mechanisms

Subfield: Inorganic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: Welch Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: G2565441525, G4405540716, Applications of Biocatalytic C–H Functionalization in Complex Molecule Synthesis