Work

Isolation, Characterization and Optimization of Indigenous Petrol Degrading Bacteria from Oil Contaminated Soil

Year: 2024

Type: article

Abstract: Petroleum-derived products like petrol, containing complex hydrocarbons, have catastrophic environmental effects, posing a universal challenge. Bioremediation is known as eco-friendly and safe technique for removal of hydrocarbon pollutants. In this study, indigenous bacteria were isolated from oil contaminated soils and screened for their biodegradation potential. The bacterial enrichment and isolation were done using selective media Bushnell-Haas supplemented with petrol as a sole carbon source. Out of fifteen isolated strains best two isolates S4P2 and S14P1 were selected on basis of DCPIP screening method. These isolates were identified as Pseudomonas chengduensis & Pseudomonas donghuensis by 16S rRNA gene sequencing. The growth parameters were optimized by one factor at a time. The optimum growth temperature for P.chengduensis & P. donghuensis was found to be 37°C at pH 7 with a substrate concentration of 1%.

Source: Journal of Pure and Applied Microbiology

DOI: 10.22207/jpam.18.3.52

Authors: Rupali D. Nanekar, Seema S. Kokitkar

Institution: University of Mumbai

Language: English

Topic: Microbial bioremediation and biosurfactants

Subfield: Pollution

Field: Environmental Science

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $500