Think twice: how single-cell suspensions from solid tissues for single-cell transcriptomics are well prepared
Year: 2024
Type: article
Abstract: Due to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues is a critical step prior to single-cell RNA sequencing. Based on a brief overview of the steps involved in preparing cell suspensions from solid tissues for single-cell RNA sequencing, we present a detailed analysis of the pivotal steps in the preparation process, including enzymatic digestion, mechanical dissociation, and cell viability evaluation. The clarification of these experimental details will greatly help us obtain high-quality single-cell sequencing data from solid tissues.
Source: Infectious Diseases & Immunity
Authors: Meng-Meng Qu, Yan-Mei Jiao
Institution: Chinese PLA General Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4402003881