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JAX-Sen: Mouse kidney dissociation for single-cell RNA sequencing v1

Year: 2024

Type: preprint

Abstract: These samples are part of the JAX-Sen project in the SENNET Consortium. We aim to study and characterize senescence in the C57Bl/6 mouse kidney. We compare young (6 months old) and old (24 months old) mouse kidneys using scRNAsequencing. This protocol describes the single-cell dissociation of the kidneys before library preparation and sequencing.

DOI: 10.17504/protocols.io.j8nlk833wl5r/v1

Authors: Ramalakshmi Ramasamy, Juliana Alcoforado Diniz, Jessica Garofalo, Patrick Fleming, Paul Robson

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4402033910

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