Consumption effect comparison of transgenic and non-transgenic soybean flour on antioxidant superoxide dismutase and spermatogenic cells profile of experimental rats
Year: 2024
Type: article
Abstract: A 90-day subchronic toxicity study was conducted to compare the consumption effect of transgenic (imported) and non-transgenic (imported and local-Grobogan) soybean flour against the superoxide dismutase (SOD) and its effect on the spermatogenic cells profile of rats. Sprague Dawley rats were given diets formulated with either 10% or 20% protein of soybean flour and compared with 10% casein protein as a control group. At the end of the study, the internal organs (kidney and liver) were collected and analyzed for the malondialdehyde (MDA) and superdioxidase (SOD) levels. The testicular tissues were embedded in paraffin and stained using an immunohistochemical technique to obtain Cu, Zn-SOD levels. The findings indicated that the consumption of various soybean types and different concentrations did not significantly affect (p>0.05) on SOD levels of the kidney and liver, nor did it significantly affect (p>0.05) on MDA levels of the kidney, but it had a significant impact on liver MDA levels. MDA and SOD levels in the kidney and liver of all groups of rats did not, however, significantly deviate from those found in earlier research. The results also showed that the administration of transgenic and non-transgenic soybean flour provided the same benefits as casein on the Cu, Zn-SOD levels in the testicular tissues. Furthermore, local-Grobogan soybean flour significantly increased the number of spermatogenic cells and Cu, Zn-SOD levels compared to all treatments. The spermatozoa profile in all groups of rats also showed no significant difference (p>0.05) in the macroscopic profile (color, pH, and consistency) and microscopic profiles (mass and individual movements, motility, abnormalities, and concentration) of spermatozoa, indicating the absence of any adverse effect in the testicular tissues of experimental rats.
Source: Food Research
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $100
OpenAlex ID: W4402066397